Establishment of a high‐efficiency transformation and genome editing method for an essential vegetable and medicine <i>Solanum nigrum</i>

Abstract Solanum nigrum , which belongs to the Solanaceae family, is an essential plant for food and medicine. It has many important secondary compounds, including glycoproteins, glycoalkaloids, polyphenolics, anthocyanin‐rich purple berries, as well ideal characteristics such self‐fertilization, a short life cycle small genome size that make it potential model study of metabolism fruit development. In this study, we report highly efficient convenient tissue culture, transformation editing method S. using leaf segments after 8 weeks with required period from initiation harvest about 3.5 months. Our results also show multi‐shoot regeneration per segment 100% shoot efficiency in medium. Moreover, over 82% kanamycin‐resistant plants exhibited strong green fluorescence marker protein expression, genetic integration confirmed by PCR expression their T 1 progeny. Furthermore, successfully applied achieve average 83% SnMYB1 gene involved regulating anthocyanin biosynthetic pathway response missing nutrients. Taken together, combination systems can provide powerful platform supporting fundamental research on molecular mechanisms metabolism, development, production compounds biotechnology.